Figure 5.
HX-MS relative difference plots for unprocessed N1-NRR (left panel) and S1 cleaved N1-NRR (right panel), comparing closed and EDTA-treated states. The differences in deuterium incorporation between the EDTA treated protein and the native protein are plotted for deuterium exchange time points of 10 seconds (blue bars); 1 minute (red bars); 10 minutes (green bars) and 4 hours (purple bars). The data shown are for peptides common to both experiments, ordered from N- to C- terminus (top to bottom). See also Figures S2 and S3, and Table S1.