Complementation assay of the wild type cricket Δ9-desaturase (AdD9des), wild type cricket Δ12/Δ9-desaturase (AdD12/9des), and cricket Δ12/Δ9-desaturase mutants 1.1, 2.1, 2.2, and 2.3 in ole1Δ. A dilution series of each sample was spotted on SC-Ura agar plates containing galactose (left) or glucose (right). Yeast cells transformed with the pYES2 vector served as a negative control. OD, optical density.