TABLE 2.
Total lipid composition of S. cerevisiae S288C cells expressing the I. galbana Δ9-elongase in combination with the A. thaliana FAD2 Δ12-desaturase (AtD12des), wild type cricket Δ12/Δ9-desaturase (AdD12/9des), or the cricket desaturase mutant 2.2
Desaturation and elongation efficiencies (%) were calculated from relative substrate and product levels (% of total fatty acids) as 100 × product/(substrate + product). Data represent means ± S.D. of three replicate cultures after a 6-, 12-, or 24-h induction.
| Fatty acid | AtD12des |
AdD12/9des |
Mutant 2.2 |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 6 h | 12 h | 24 h | 6 h | 12 h | 24 h | 6 h | 12 h | 24 h | |
| 16:0 | 9.9 ± 0.3 | 7.3 ± 0.2 | 5.8 ± 0.1 | 10.1 ± 0.6 | 7.0 ± 0.4 | 5.2 ± 0.2 | 10.5 ± 0.2 | 8.2 ± 0.3 | 5.6 ± 0.1 |
| 16:1Δ9 | 25.7 ± 0.5 | 25.7 ± 0.4 | 21.7 ± 0.3 | 26.6 ± 1.4 | 26.5 ± 0.1 | 22.9 ± 0.2 | 25.5 ± 0.1 | 27.5 ± 1.4 | 22.9 ± 0.5 |
| 18:0 | 9.3 ± 0.2 | 8.8 ± 0.3 | 11.1 ± 0.1 | 9.0 ± 0.3 | 8.3 ± 0.1 | 9.8 ± 0.3 | 9.5 ± 0.1 | 8.6 ± 0.4 | 9.7 ± 0.1 |
| 18:1Δ9 | 53.2 ± 0.8 | 50.1 ± 0.6 | 41.9 ± 2.3 | 52.7 ± 1.7 | 55.9 ± 0.3 | 58.2 ± 0.7 | 52.7 ± 0.4 | 51.8 ± 1.7 | 51.5 ± 1.1 |
| 18:2Δ9,12 | 0.4 ± 0.0 | 6.0 ± 0.9 | 14.9 ± 2.1 | 0.1 ± 0.0 | 0.3 ± 0.0 | 1.2 ± 0.0 | 0.2 ± 0.0 | 1.2 ± 0.4 | 4.5 ± 0.1 |
| 20:2Δ11,14 | 0.0 ± 0.0 | 0.4 ± 0.1 | 3.2 ± 0.3 | 0.0 ± 0.0 | 0.2 ± 0.1 | 1.0 ± 0.1 | 0.0 ± 0.0 | 1.1 ± 0.3 | 4.3 ± 0.3 |
| Other | 1.5 | 1.7 | 1.5 | 1.6 | 1.7 | 1.7 | 1.6 | 1.7 | 1.6 |
| % of desaturation | 0.7 | 11.3 | 30.2 | 0.2 | 0.9 | 3.6 | 0.4 | 4.3 | 14.6 |
| % of elongation | 0.0 | 6.3 | 17.7 | 0.0 | 40 | 45.5 | 0.0 | 47.8 | 48.9 |