TABLE 3.
Fatty acid composition of PC and NL fractions of S. cerevisiae S288C cells expressing the I. galbana Δ9-elongase in combination with the A. thaliana FAD2 Δ12-desaturase (AtD12des), wild type cricket Δ12/Δ9-desaturase (AdD12/9des), or the cricket desaturase mutant 2.2
Data represent means ± S.D. of three replicate cultures after a 24-h induction.
| Fatty acid | AtD12des |
AdD12/9des |
Mutant 2.2 |
|||
|---|---|---|---|---|---|---|
| NL | PC | NL | PC | NL | PC | |
| 16:0 | 4.3 ± 0.1 | 6.9 ± 0.8 | 4.0 ± 0.3 | 3.5 ± 0.3 | 4.4 ± 0.7 | 3.8 ± 0.1 |
| 16:1Δ9 | 20.4 ± 0.2 | 42.2 ± 1.6 | 20.5 ± 0.6 | 41.5 ± 1.3 | 21.2 ± 1.1 | 41.9 ± 1.4 |
| 18:0 | 12.8 ± 0.8 | 7.7 ± 0.8 | 11.0 ± 0.4 | 4.8 ± 2.0 | 11.0 ± 1.3 | 4.2 ± 0.8 |
| 18:1Δ9 | 45.1 ± 1.5 | 22.0 ± 2.9 | 54.9 ± 2.0 | 47.0 ± 1.8 | 49.0 ± 2.0 | 43.4 ± 0.8 |
| 18:2Δ9,12 | 10.0 ± 1.3 | 17.9 ± 4.3 | 1.1 ± 0.1 | 1.1 ± 0.3 | 4.1 ± 0.2 | 3.0 ± 0.3 |
| 20:2Δ11,14 | 3.9 ± 0.4 | 1.4 ± 0.4 | 1.2 ± 0.1 | 0.4 ± 0.3 | 5.4 ± 0.6 | 1.7 ± 0.0 |
| Other | 3.5 | 1.9 | 7.3 | 1.7 | 4.8 | 1.9 |