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. 2011 Feb 18;286(15):13011–13022. doi: 10.1074/jbc.M110.169813

TABLE 1.

Scanning density analysis of SDS-PAGE bands using Image Quantity Software TL (band intensity, percentage of total detected protein stain in each sample, n = 3)

The density of each band is compared with the integrated total of all the bands (and diffuse staining) in that individual sample. For any individual sample, the sum of staining from the α1, α2, β, and γ bands will be less than 100%; the remainder represents the diffuse staining in other Mr regions of that sample.

α2 α1 β γ
Collagen I 10.75 ± 0.39 24.37 ± 0.43 27.36 ± 0.60 23.06 ± 0.16
Collagen I + RFUVA 5.89 ± 0.41 10.99 ± 0.39 15.34 ± 0.61 44.47 ± 1.07
Collagen I + KER + RFUVA 7.71 ± 0.16 17.63 ± 0.29 32.56 ± 0.76 42.34 ± 0.79
Collagen I + LUM + RFUVA 7.58 ± 0.19 19.13 ± 0.41 28.21 ± 0.23 34.83 ± 0.78
Collagen I + MIM + RFUVA 3.22 ± 0.22 7.28 ± 0.20 11.76 ± 0.58 54.07 ± 0.61
Collagen I + DEC + RFUVA 2.25 ± 0.28 5.11 ± 0.22 15.11 ± 0.35 59.86 ± 1.69