FIGURE 5.
AS discriminates lipid phase. A, fluorescence spectra of AS-18MFE (200 nm) in the presence of DMPC SUVs (0.2 mm) as a function of the temperature. The sharp increase corresponds to the range of membrane melting temperature (23 °C). B, temperature dependence of the integral fluorescence intensities of AS-18MFE in buffer (□) and in the presence of DPPG (♢, Mt ∼ 41 °C), DMPC (△, Mt ∼ 23 °C), and DPPC (○, Mt ∼ 41 °C) SUVs. The experiment was performed by decreasing the temperature of a solution. All measurements were performed in 25 mm Na-PO4, pH 6.2, 150 mm NaCl. SUV diameter was 40 ± 5 nm. a.u., arbitrary units.