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Alignment of structures and sequences of BCCP fragments. A, superimposition of PhBCCP (gray, 2EJG), HsBCCP (dark gray, 2JKU), and EcBCCP (light gray, 1A6X) structures generated with PyMOL software. B, sequence alignment of the three BCCPs, generated using Jalview (33, 34). The working HsBCCP sequence has an exogenous tyrosine residue at the N terminus, which was added in subcloning to allow for spectrophotometric protein concentration determination.
