TABLE 1.

Biotin transfer rates for cognate ligase-BCCP pairs

Pair	Stopped-flowa	Quench-flowb
	m−1s−1	m−1s−1
HsBPL	35,000 ± 3,000	31,000 ± 3,000
EcBPL	11,500 ± 700	10,500 ± 800
PhBPL	15,000 ± 600c	15,000 ± 1000c

^a The errors represent the S.D. of three independent stopped-flow experiments.

^b The errors correspond to the S.D. of results obtained in two independent quench-flow experiments.

^c Measurements with PhBPL were carried out at 40 °C, in 10 mm Tris, pH 7.5, 500 mm KCl, 2.5 mm MgCl₂. All other experiments were performed at 20 °C, in 10 mm Tris, pH 7.5, 200 mm KCl, 2.5 mm MgCl₂.