FIGURE 3.

Nascent HDL synthesis and cholesterol efflux to exogenous apoA-I by hLrp1^+/+ and hLrp1^−/− hepatocytes. Endogenous cholesterol in primary hepatocytes (400,000 cells/well) from hLrp1^+/+ and hLrp1^−/− mice were prelabeled by incubation for 16 h with 10 μCi/ml [³H]mevalonate. Nascent HDL synthesis (A) and exogenous apoA-I-mediated cholesterol efflux (B) were determined by measuring anti-apoA-I-immunoprecipitable [³H]cholesterol secreted into the culture medium after incubation for 6 h in the absence or presence of exogenously added apoA-I, respectively. The data represent mean ± S.E. from six mice per group in two separate experiments. * indicates statistical significant difference at p < 0.05.