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. 2011 Feb 22;286(15):13079–13087. doi: 10.1074/jbc.M111.229369

FIGURE 4.

FIGURE 4.

Apolipoprotein secretion and lipoprotein receptor expression in hLrp1+/+ and hLrp1−/− hepatocytes. Primary hepatocytes from hLrp1−/− and hLrp1+/+ mice were cultured in serum-free medium for 20 h. A, equal volumes of conditioned media were resolved by SDS-polyacrylamide gels and immunoblotted using antibodies against apoA-I and apoE. B, equal protein concentrations of whole cell lysates were immunoblotted with antibodies against ABCA1. C, cell surface proteins were labeled with sulfo-NHS-SS-biotin and then precipitated using immobilized streptavidin. The precipitates were electrophoretically resolved and immunoblotted using antibodies against the 515-kDa subunit of LRP1, ABCA1, SR-BI, LDL receptor (LDLR), and VLDL receptor (VLDLR). Each lane in the immunoblots represents a sample from an individual well. The immunoblots were scanned, and the intensity of the bands was quantified by image analysis. The average pixel density of the bands from hLrp1+/+ hepatocyte cultures was arbitrarily set at 1.0 in each case. The error bars indicate S.D. from the mean values with * indicating significant differences from the hLrp1+/+ groups at p < 0.01.