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Western blot analysis of protein expression in human melanoma cell lines. Cell extracts were made as described under “Experimental Procedures.” Upper panel, immunotransfers were blotted with anti-B-Raf antibody. 2nd to 4th panels were immunoblotted with anti NHE1, anti-phospho-ERK1/2, and anti ERK1/2 antibodies, respectively. Results are typical of three independent experiments.
