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. 2011 Feb 23;286(15):13096–13105. doi: 10.1074/jbc.M110.165134

FIGURE 7.

FIGURE 7.

Effect of siRNA on levels of B-Raf and NHE1 in HeLa and Mel2a cells. A, Western blot analysis of effects of siRNA on B-Raf protein and phospho-ERK1/2 levels. Upper panel, mock (Mk), 200 nm nonspecific (NS), and 200 nm B-Raf siRNA (si)-treated cells immunoblotted for B-Raf protein levels. Middle panel, effect of varying concentrations of B-Raf siRNA or nonspecific siRNA on B-Raf levels of Mel2a cells. Lower panels, effect of B-Raf siRNA on phospho-ERK1/2 (pERK1/2) and ERK1/2 levels in Mel2a cells. B, effect of siRNA on NHE1 activity in HeLa and Mel2a cells. An acute acid load was induced in cells either treated with 200 nm of nonspecific or B-Raf siRNA. The rate of recovery from the acid load was compared in the two groups. Results are mean ± S.E. of at least six experiments. * indicates significantly different from the rate of recovery with nonspecific treatment at p < 0.005.