FIGURE 3.

Amino acids 145–243 of SF3a120 are sufficient for SF3a60 binding. A, scheme of in vitro-translated SF3a120 proteins. Protein domains are as defined in the legend to Fig. 1. B, SDS-PAGE and Coomassie Blue staining of recombinant GST (lane 1) and GST-3a60-FL (lane 2). C, GST pulldown of N- and C-terminal SF3a120 deletion mutants by GST-3a60-FL. The experiment was performed as described in Fig. 1B. D, GST pulldown of internal SF3a120 deletion mutants with GST-3a60-FL. The experiment was performed as in C, except that proteins were separated by 15% SDS-PAGE.