TABLE 1.

Predicted TMDs in yeast, murine, and tung tree DGAT2s

The polypeptides corresponding to yeast (S. cerevisiae), murine (M. musculus), and tung tree (V. fordii) DGAT2s were submitted to seven transmembrane prediction algorithms.

	HMMTOP	TMHMM	TopPred	SVMtm	SOSUI	ConPred II	TMpred
ScDGAT2	68–92	70–92	68–88	66–97	75–97	73–93	72–92
				193–207	188–210	189–209	196–214
	200–224		215–235	217–231	213–234	215–235	216–236
	294–310		290–310			293–313
	341–359
No. of TMD	4	1	3	3	3	4	3
Orientationa	IN	IN	IN	NA	NA	IN	IN
MmDGAT2	68–92	73–95	76–96	73–93	66–88	70–90	76–96
				95–109	93–115
			230–250				224–248
No. of TMD	1	1	2	2	2	1	2
Orientationa	IN	IN	IN	NA	NA	IN	IN
VfDGAT2					14–36
	29–47	24–46	28–48	27–52		28–48	25–46
	52–70	50–72	53–73	54–69	47–69	51–71	50–72
	121–139	112–134	119–139	122–136		119–139	121–139
	170–187		168–188			169–189	170–193
	208–226		211–231				211–231
	247–265		211–231			246–266	250–273
No. of TMD	6	3	6	3	2	5	6
Orientationa	IN	IN	IN	NA	NA	IN	IN

^a Orientation was of the N terminus. Cytosol, IN.