TABLE 2.

OAADPr deacetylation activity of selected wild-type and mutant MacroD-like proteins

The activity below 0.005 s⁻¹ is considered background or inactive under the assay conditions.

Proteins	Organism	Mutations	Catalytic activity, kcat
			s−1
MacroD1	Human	Wild type	0.198 (100%)
MacroD1	Human	D184A	0.078 (∼39.3% of wt activity)
MacroD1	Human	H188A	0.114 (∼57.6% of wt activity)
MacroD1	Human	D167A	0.136 (∼68.7% of wt activity)
MacroD1	Human	N171A	0.082 (∼41.3% of wt activity)
MacroD1	Human	N174A	0.113 (∼57.3% of wt activity)
MacroD1	Human	S176A	0.195 (∼98.4% of wt activity)
MacroD1	Human	C199A	0.179 (∼90.4% of wt activity)
MacroD1	Human	T226A	0.204 (∼102.9% of wt activity)
MacroD1	Human	D160A	0.114 (∼57% of wt activity)
MacroD1	Human	S268A	0.153 (∼77% of wt activity)
MacroD1	Human	N174A + D184A	0.014 (∼7% of wt activity)
MacroD1	Human	G270E	0.002 (∼1% of wt activity)
YmdB	E. coli	Wild type	0.48 (100%)
YmdB	E. coli	G124E	0.005 (∼1.0% of wt activity)
SAV0325	S. aureus	Wild type	2.77 (100%)
SAV0325	S. aureus	G214E	0.007 (∼0.2% of wt activity)
SAV0325	S. aureus	D122A	0.679 (∼24.5% wt activity)