FIGURE 2.

A, FGFR3 and Neu constructs used in the study. The abbreviations used are as follows: EC, extracellular domain; TM, transmembrane domain; JM, juxtamembrane domain; KIN, kinase domain. B, immunofluorescence staining of the truncated FGFR3 constructs (ECTM_WT-1, ECTM_WT-2, and ECTM_WT-3) and the NeuECTM construct, expressed in HEK 293 cells. Intact cells were stained with anti-N-FGFR3 or anti-N-Neu antibodies without cell permeabilization. These antibodies recognize the extracellular domains of the truncated constructs. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L). Scale bar, 100 μm. C, expression levels of truncated FGFR3 and Neu, as detected by Western blots, probed by anti-N-FGFR3 antibodies. Actin staining served as a loading control. 1, empty vector; 2, ECTM_WT-1; 3, ECTM_WT-2; 4, ECTM_WT-3; and 5, Neu-ECTM.