FIGURE 5.
Effect of ECTMWT-1 on FGFR3/WT phosphorylation in stable cell lines, as a function of ligand (FGF1) concentration. HEK 293 cells stably expressing FGFR3/WT were transfected with plasmids encoding ECTMWT-1 or an empty vector. Cells were stimulated with different concentrations of FGF1, ranging from 1 to 2500 ng/ml. The phosphorylation of FGFR3/WT in the stable cells was detected using anti-p-FGFR3 antibodies, and the total expression level of FGFR3 was probed by anti-C-FGFR3 antibodies, recognizing the intracellular domain of FGFR3 and thus not reactive to the truncated ECTMWT-1.