FIGURE 6.

Effect of mutations in the E-loop of Nox4 on Erk1/2 phosphorylation. HEK293 cells were transiently transfected with the plasmids indicated and subsequently subjected to SDS-PAGE and Western blot analysis with the antibodies indicated. A and B, representative blots (A) and statistical analysis (B) of the ratio of phosphorylated to non-phosphorylated Erk1/2 (pERK1/2). To allow better comparison, the signal of wild-type Nox4 was set to 100%. n ≥ 3, mean ± S.E. *, p < 0.05 versus Nox4 construct. % of Max., percentage of maximum.