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Effect of PA on the kinetic behavior of SPHK1. A, SPHK1 activity measured as a function of phytosphingosine molar concentrations at set mol fractions of PA. The molar fraction of phytosphingosine was 0.01. Data represent the average of three replicates. B, SPHK1 activity measured as a function of phytosphingosine mol fractions at set mol fractions of PA. The molar concentration of phytosphingosine was 50 μm. Data represent the average of three replicates.
