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. 2011 Feb 25;286(15):13346–13356. doi: 10.1074/jbc.M110.216416

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — TAP transport activity depends on the lipid composition. A and B, steady-state kinetics of peptide transport by reconstituted TAP. TAP was reconstituted into liposomes prepared from E. coli total lipids, and peptide transport was measured in the presence of varying concentrations of peptide (A) or ATP (B). Data were fitted to the Michaelis-Menten equation. C, cholesterol inhibits TAP activity. TAP was reconstituted into liposomes prepared with different lipid mixtures, and transport activity was determined. D, transport activity of reconstituted TAP depends on specific lipids. Activity of TAP reconstituted into liposomes made from most abundant phospholipids of the ER was analyzed as in C. Ratios of ER lipids are DOPC/DOPE/DOPS/PI/lyso-PC/sphingomyelin (61:19:3:9:4.5:5). Error bars show S.D. Asterisk, no transport detectable. Chol, cholesterol.