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Effect of a stepwise increment of extracellular glucose concentrations on YFP/CFP ratio. Representative glucose-dependent YFP/CFP ratio changes are shown in a fibroblast (A), in a preadipocyte (B), and in an adipocyte (C). D, a representative recording of adipocytes with no response to extracellular glucose (in 86% of cells). Numbers under the gray step-like traces indicate the concentration of extracellular glucose (in mm). Scale bars on all images: 20 μm.
