FIGURE 2.

Expression of Ano1 transcriptional variants is altered in diabetic gastroparesis. A, schematic of the 5′ end of Ano1. Segment a (exon 1 and the 5′ region of exon 2) is indicated in black. B and C, the expression of transcripts that contain segment a (comprised of exons 1 and 2) was significantly reduced (B), whereas the expression of exon 6b was increased in patients with diabetic gastroparesis compared with controls (C). D and E, no difference was detected for the expression of exons 13 (D) and 15 (E). F, graph shows lower expression of mRNA for segment a in patients with type 1 diabetes versus type 2. In each graph, the circles represent individual patients, and the lines are the median values; the data are expressed as ratios to the total Ano1 in each sample. *, p < 0.004; **, p < 0.05 (Mann-Whitney test).