FIGURE 3.

Effects of charge reversal mutations on the outward conductance of α1β heteromeric Gly receptors expressed by coinjection of the α1 and β subunit constructs, or constrained to contain two α and three β subunits by means of concatenated constructs. (α1_β) tandem subunit constructs were co-injected with a monomeric β subunit into Xenopus oocytes and the outward currents were recorded at different voltages. A shows examples of traces recorded at positive voltages for different combinations of wild-type and mutated subunits (display f_c = 1 kHz). Slope conductance values for each combination of mutant and wild-type subunits are reported in Table 3. B shows current-voltage plots for wild type and mutant receptors. α1β (Δ), α1^KEβ (●), α1β^KE (▿),α1^KEβ^KE (□). Mutations carried by the β subunit had no effect on conductance, even though the stoichiometry of this receptor is 2α:3β. C shows examples of traces for the same wild type and mutant combinations expressed by co-injecting un-tethered α1 and β subunits. D shows current-voltage plots for wild-type and mutant un-tethered receptors.