TABLE 1.
Solutions used throughout the recordings
Glycine receptor conductances were measured using S1 and S2 solutions for negative and positive voltages respectively. Due to the low conductance of GABAA receptors we bathed the cells with S2 solution in combination with S3 in the recording pipette to obtain the maximal achievable conductance (12). Gly 200 μm and GABA 10 mm were the concentration of agonists added to the pipette solution. Concentrations are expressed in mm.
| Compound | Stripping solution | S1 | S2 | S3 | S4 |
|---|---|---|---|---|---|
| Na-gluconate | 20 | 5.4 | |||
| K-gluconate | 200 | ||||
| NaCl | 102.7 | 5.4 | 5.4 | ||
| KCl | 20 | 2 | 142 | 170 | 273.6 |
| CaCl2 | 2 | 2 | 2 | 2 | |
| MgCl2 | 1 | 1 | 1 | 1 | 1 |
| HEPES | 10 | 10 | 10 | 10 | |
| EGTA | 10 | ||||
| MES | 10 | ||||
| TEA-Cl | 20 | 20 | 20 | ||
| Sucrose | 15 | 15 | |||
| pH | 7.6 | 7.4 | 7.4 | 6.0 | 7.4 |