FIGURE 3.

Kinetics of the pressure-induced dissociation of ThT from the amyloid fibrils. A, shown are residuals of the pressure-induced relaxation kinetics after fitting the data to a single and double exponential equation. Temperature (B) and pressure (C) dependence of the pressure-induced amyloid structural kinetics is shown. Pressure-jumps (from 0.1 to 600 MPa) were carried out at different temperatures; 15 °C (solid line), 25 °C (long dashed line), 35 °C (short dashed line), and 45 °C (dashed and dotted line). Pressure-jumps (at 25 °C) were performed to obtain different final pressures from 10 to 600 MPa (short and long dashed line), to 540 MPa (dashed and dotted line), to 470 MPa (short dashed line), to 400 MPa (long dashed line), and to 330 MPa (solid line). Insets show the pressure and temperature dependence of the observed relaxation times (τ) calculated from the fast phase (solid circles) and from the slow phase (open circles). Solid lines are linear fits to the data.