FIGURE 2.

HO-1 partially mediates the inhibitory effects of flAcrp on MyD88-dependent and -independent cytokine signatures in RAW264.7 macrophages. A and B, RAW264.7 cells were cultured for 18 h in the absence or presence of 0.5 μm zinc protoporphyrin (ZnPP) in the presence or absence of flAcrp (45 nm). Cells were then stimulated with 100 ng/ml LPS for 4 h and expression of TNFα and IL-6 mRNA (A) and IFN-β and CXCL10 mRNA (B) were normalized to 18 S mRNA. C and D, RAW264.7 cells were transfected or not with 100 nm of HO-1 siRNA or scrambled siRNA and then cultured with or without 45 nm flAcrp for 18 h. Cells were then stimulated with LPS for 4 h. TNFα and IL-6 mRNA (C) and IFN-β and CXCL10 mRNA (D) were normalized to 18 S mRNA. n = 4. LPS-stimulated cells with different superscripts (a–c) are significantly different from each other (p < 0.05).