FIGURE 6.
Phosphorylation promotes Shh signaling-induced co-localization of Sufu and Gli3 in primary cilia. Representative immunofluorescent staining of Gli3 (A) and quantification thereof (B) show Shh signaling-induced localization at the tip of the primary cilia in SmoFlox/Flox MEFs and curtailment by Ad-cre infection as in Fig. 3, A and B. C and D, effects of cyclopamine and KT5720 treatment on the percentage of Gli3 positive primary cilia and the intensity of Gli3, respectively. E, representative immunofluorescent staining and quantification of Gli3 (F) restored by Sufu and its phosphorylation site mutants that were force-expressed in Sufu−/− cells. *, p < 0.01. G, model for the regulation of Shh-induced co-localization of Sufu and Gli2/3 in primary cilia by phosphorylation. Purm, purmorphamine.