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Sequential phosphorylation of the sst2A receptor. CHO-sst2A cells were stimulated with 10 nm SS14 at 37 °C for the times shown. Cells were then fixed, and receptor phosphorylation was measured at Ser-341/343 (panel A) or Thr-353/354 (panel B) using the in-cell ELISA. The half-times for receptor phosphorylation at Ser(P)-341/343 and Thr(P)-353/354 were 0.44 ± 0.13 and 2.11 ± 0.33 min, respectively (mean ± S.E., n = 4), and were significantly different (p < 0.01).
