Skip to main content
. 2011 Feb 22;286(15):13561–13573. doi: 10.1074/jbc.M110.215723

FIGURE 5.

FIGURE 5.

Duration of agonist stimulation determines subsequent receptor dephosphorylation. CHO-sst2A cells were stimulated at 37 °C with 10 nm SS14 for either 2 min (panel A) or 15 min (panel B). Following a rapid wash, cells were incubated in fresh 37 °C medium without agonist for the times shown. Receptor phosphorylation was determined at Ser-341/343 (●) and Thr-353/354 (○) using the in-cell ELISA. Nonlinear regression curve fitting to a single exponential decay in multiple independent experiments gave a half-time for receptor dephosphorylation at Thr-353/354 of 6.5 ± 0.5 min (n = 5) after 2 min of SS14 treatment and 7.5 ± 0.5 min (n = 4) after 15 min of SS14 stimulation. The half-time for Ser-341/343 dephosphorylation could not be calculated after 2 min of SS14 treatment because so little of the receptor was dephosphorylated. The half-time for Ser-341/343 dephosphorylation following a 15-min SS14 challenge was 6.7 ± 0.6 min (n = 4).