FIGURE 12.

Proliferation assay using antibodies as inhibitors. Anti-VEGF(102–122), P3(NC), and P3(CYC) correspond to polyclonal antibodies against peptides MVF-VEGF(102–122), MVF-VEGF-P3(NC), and MVF-VEGF-P3(CYC) raised in our laboratory. Antibodies were purified from bleed 3Y+2 or 3Y+3. Rabbit IgG, rabbit total IgG; mAb anti-VEGF, monoclonal antibody against human VEGF protein (R&D Systems) shown to block proliferation. The results represent average from two set of experiments, each one done in triplicate and is expressed as inhibition percentage relative to the control (VEGF stimulated cells without inhibitors). Error bars represent mean ± S.E.