FIGURE 6.

A, inhibitory effects of peptides on HUVEC proliferation; B, network formation in Matrigel. The results represent an average from two set of experiments, each one done in triplicate, and are expressed as inhibition percent relative to the control (VEGF-stimulated cells without inhibitors). Pictures at ×40 magnification from light microscopy are shown for the Matrigel assay (B), and average sprout points counted using the software ImageJ (National Institutes of Health) are shown in C. Inhibitors were used at the indicated concentration. KDR-Fc is an extracellular domain from VEGFR2, and SU1498 (Calbiochem) is the small molecule tyrosine kinase inhibitor. ECGS represents a positive control where growth condition was used, i.e. medium supplemented with 20% FBS and ECGS. Irrelevant peptide represents a scrambled sequence with similar molecular weight. * represent p < 0.05 using Student's t test, indicating that only cells treated with irrelevant peptide were not significantly different from the control. Error bars represent mean ± S.E.