Inhibition of VEGF-dependent angiogenesis in Matrigel plugs by VEGF peptides. Top panel, 1st lane, Matrigel containing PBS alone (−control); 2nd lane, VEGF (+ control); 3rd lane, VEGF + P3 peptides; 4th lane, VEGF + P4, and 5th lane, VEGF + irrelevant peptide (VEGF + IRR) were subcutaneously administered to BALB/c mice on the right and left flanks, and 10 days later the plugs were removed and photos taken as shown. Bottom panel, hemoglobin content was determined using Drabskin's method. Each group contained three mice. Error bars represent mean ± S.D.