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TM induces apoptosis in L. major. A, percentage of viable cells after treatment of 0.2% DMSO as control or TM. Promastigotes of L. major were treated at the indicated time, and parasite viability was detected by MTT assay. Average and S.D. values were taken from three independent experiments and plotted against time. B, flow cytometric analysis of PS externalization in control or TM-treated cells. C, TUNEL analysis by flow cytometry.
