FIGURE 2.

TM induces unfolded protein response in L. major. A, Western blot analysis of lysates prepared from DMSO-treated control (1st lane) or TM-treated cells (2nd to 5th lanes). 30 μg of total protein was resolved by SDS-PAGE and probed with T. brucei anti-Bip antibody or α-tubulin antibody as loading control. The numbers on the lanes indicate the hours of treatment. B, densitometric analysis of the blot showing up to 4-fold up-regulations of Bip after TM treatment. The asterisks indicate the level of statistical significance (0.05).