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TM induces ROS generation. A, time course kinetic analysis of intracellular ROS generation by H2DCFDA for the promastigotes treated with 0.2% DMSO alone, 20 μg/ml TM, or after preincubation with NAC. B, flow cytometric analysis of mitochondrial ROS generation by MitoSOXTM. Data are representative of at least three independent experiments.
