FIGURE 7.

Analysis of ER stress-induced apoptosis in L. major in presence of different inhibitors. A, flow cytometric analysis of PS exposure in DMSO-treated control cells, 16 h in TM-treated cells, and cells pretreated with NAC, GSH, l-cysteine, BAPTA-AM, PBA (chemical chaperone), antipain, and Z-VAD-FMK. Lower right and upper right quadrants of each dot plot represent apoptotic cells. B, flow cytometric analysis of DNA fragmentation by TUNEL assay. Lower right and upper right quadrants of each dot plot represent TUNEL-positive apoptotic cells. Data are representative of three independent experiments.