TABLE 1.
Measurement of caspase-like activity in control and TM-treated L. major cells
An increased fluorescence intensity indicates increased caspase-like protease activity. Measurements were described under “Experimental Procedures.” Each value represents the means ± S.D. for three independent experiments. FLICA indicates a caspase-3- and -7-specific fluorogenic inhibitor.
| Cells | Caspase-12-like activity |
Caspase 3/7-like activity |
Metacaspase-like activity |
|||
|---|---|---|---|---|---|---|
| ATAD-AFC | ATAD-AFC and Z-ATAD-FMK | FLICA | FLICA and VAD-FMK | Boc-GRR-AMC | Boc-GRR-AMC and antipain | |
| Control | 18757 ± 1578 | 18353 ± 1218 | 162 ± 11 | 122 ± 14 | 409 ± 30 | 338 ± 29 |
| TM treated | 21676 ± 1131 | 19912 ± 2078 | 231 ± 17 | 176 ± 9 | 658 ± 21 | 425 ± 32 |