FIGURE 2.

Genomic instability and sister chromatid exchange in cells with BRCA1-RAP80 deficiency. A, metaphases were collected from cells treated with RAP80 siRNA and analyzed for chromosome and chromatid breaks. Cells treated with RAP80 siRNA show an increase in chromatid breaks. Data are representative of three independent experiments. See supplemental Fig. S2C for an independent replicate experiment. B, representative images of SCE following knockdown of RAP80 or BRCC36 and subsequent etoposide treatment. C, quantification of images as in B. Cells treated with RAP80 or BRCC36 siRNA show a significant increase in etoposide-induced SCE formation. Error bars, S.E. from three independent experiments. p values were calculated in comparison with control siRNA-treated cells. D, clonogenic survival assay of cells treated with the indicated siRNAs after exposure to different doses of etoposide. Error bars, S.D. See supplemental Fig. S2F for an independent replicate experiment.