TABLE 1.

Characteristics of filopodia induced by Rif and mDia1

N1E115 cells were cotransfected with cDNA for fluorescence-tagged actin or actin-binding peptide and the protein of interest. Time lapse imaging of fluorescent cells was done at 18 h post-transfection, and the number of filopodia formed per cell and the length and lifetime of such protrusions were measured (see “Experimental Procedures” for details). Endogenous, filopodia seen when cells were transfected with only GFP-actin; RifQL, cells cotransfected with untagged RifQL and GFP-actin; mDia1, cells cotransfected with EYFP-mDia1 and mCherry-Abp140p; mDia2, cells cotransfected with Myc-mDia2 and GFP-actin; mDia2H160D, cells cotransfected with Myc-mDia2H160D and GFP-actin; Rif + mDia2H160D, cells cotransfected with untagged RifQL, Myc-mDia2H160D, and GFP-actin. Data are presented as mean ± S.E. For endogenous, n = 39; RifQL, n = 23; mDia1, n = 18; mDia2, n = 18; mDia2H160D, n = 20; Rif + mDia2H160D, n = 20.

Protein(s) expressed	Filopodia/cell	Length	Lifetime
		μm	s
Endogenous	1.2 ± 1.02	7.16 ± 0.82	212 ± 64
IRSp53a		6.80 ± 1.88	187 ± 38
N-WASPa		7.40 ± 0.97	154 ± 20
RifQL	8.5 ± 2.36b	4.37 ± 0.28c	162 ± 14
mDia1	5.6 ± 1.27d	3.99 ± 0.60c	135 ± 19
mDia2	6.5 ± 0.73b	4.61 ± 1.76	163 ± 78
mDia2H160D	9.3 ± 2.61b	3.46 ± 0.12d	137 ± 42
Rif + mDia2H160D	7.0 ± 2.42d	3.35 ± 0.17d	135 ± 34

^a Data for length and lifetime of IRSp53 and N-WASP filopodia in N1E115 cells from Lim et al. (8) are shown in italic type for comparison but excluded from the statistical analysis.

^b p < 0.001 with respect to the values for “endogenous” in the same column.

^c p < 0.05 with respect to the values for “endogenous” in the same column.

^d p < 0.01 with respect to the values for “endogenous” in the same column.