FIGURE 6.

Survival rate of mice and the change of bone marrow with varying ⁶⁰Co γ-ray irradiation and the survival rate of mice and the aGVHD clinical and histopathological scores after transplantation. A, Kaplan-Meier curve for mice that received varying doses of ⁶⁰Co γ-ray irradiation. All of the mice that received 8.0 Gy died within 14 days; all of the mice that experienced 5.0 Gy and 6.0 Gy ⁶⁰Co γ-ray irradiation lived more than 30 days, and 1 of 10 mice died after receiving 7.0 Gy ⁶⁰Co γ-ray. The other living mice receiving 7.0 Gy ⁶⁰Co γ-ray lived more than 30 days. B, bone marrow biopsies of mice that experienced varying doses of ⁶⁰Co γ-ray irradiation (a, 5.0 Gy; b, 6.0 Gy; c, 7.0 Gy; d, 8.0 Gy; e, normal mice). The hematopoietic tissues were extremely rare in the mice that received 8.0 Gy ⁶⁰Co γ-ray irradiation. Although the hematopoietic tissues decreased in other mice, this decrease was not obvious. All of the mice experienced hematopoietic recovery after 14 days. However, the hematopoietic tissues in the mice that received 7.0 Gy were the least relative to the mice that received 5.0, 6.0, and 7.0 Gy ⁶⁰Co γ-ray irradiation. Scale bars, 10 μm C, Kaplan-Meier curve for mice after transplantation. All of the mice from untreated group survived more than 30 days. 2 of 20 mice died within 30 days for total body irradiation group. 7 of the 20 mice in the BMC group died within 30 days, and 4 mice died in BMT + hUCBDSCs group. All the mice from the BMT group developed moderate to severe GVHD clinical signs such as weight loss, hunched posture, and ruffled fur, and 80% of the mice survived for 30 days. However, the percentage of survival in the BMT + hUCBDSCs group was significantly increased (23%) compared with BMT group (p < 0.05). D, clinical scores after transplantation. The GVHD clinical scores of BMT + hUCBDSCs group were significantly lower than those of BMT group on days 7, 14, 21, and 28 after transplantation (p < 0.05). E, histopathological score after transplantation. The histopathological score in BMT + hUCBDSCs group was significantly lower than that in BMT group (p < 0.05). 20 mice were used in each group, and 3 mice were used at each time point.