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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Traffic. 2010 Dec 22;12(2):170–184. doi: 10.1111/j.1600-0854.2010.01145.x

Figure 6. Analysis of the proteolytic fragmentation of lysine-mutant DORs show delayed destruction specifically of the receptor endodomain.

Figure 6

HEK293 cells stably expressing F-DOR-0cK-HA were incubated with 10μM DADLE for the indicated time period before lysis. HA-linked fragments were immunoprecipitated, treated with PNGaseF, and separated via SDS-PAGE. Shown is a representative anti-HA blot (A). Proteolytic fragments resolved to the indicated sizes, corresponding topologically as diagrammed in (B).