Table II. BGL-1 enzyme yield in transplastomic tobacco plants.
One unit of BGL-1 enzyme is defined as the amount of enzyme that released 1 μmol of p-nitrophenol from pNPG substrate under the assay conditions described in “Materials and Methods.” Enzyme yield per acre per year was determined using the following information: 1,5941.4 BGL-1 units per plant × 8,000 plants per acre × three cuttings per year = 382.59 million units per acre per year.
Enzyme | Leaf Age | No. of Leaves per Plant | Average Weight per Leaf | Units g−1 in Leaf | Units |
Whole Plant Yield | Units (Millions) per Acre per Cutting | Units (Millions) per Acre per Year | |
Per Leaf | Per Age Group | ||||||||
g | units | ||||||||
BGL-1 | Young | 5.7 | 7.1 | 9.24 | 65.6 | 373.92 | 1,5941.4 | 127.53 | 382.59 |
Mature | 14.2 | 16.5 | 44.41 | 732.77 | 10,405.33 | ||||
Old | 8.2 | 14.9 | 42.25 | 629.53 | 5,126.15 |