Table IV. Division and plating efficiency of protoplasts derived from BGL-1 and UT.

Hormone Combination (mg L−1)	No. of Protoplasts Observed	No. of Divisions	Division Efficiencya	No. of Protoplasts Observed	No. of Calli	Plating Efficiencyb
			%			%
Free hormone
UT	382	0	0	532	0	0
	428	0		432	0
	469	0		470	0
BGL-1	482	17	2.8 ± 0.7	411	9	2.0 ± 0.3
	478	13		485	11
	467	10		421	7
Zeatin (1.0) + naphthylacetic acid (3.0)
UT	510	134	28.0 ± 1.7	531	51	9.1 ± 0.6
	514	145		504	47
	481	142		469	40
BGL-1	412	141	34.0 ± 2.0	410	62	14.1 ± 1.4
	514	185		519	77
	489	156		481	60
Naphthylacetic acid (3.0)
UT	397	22	4.8 ± 0.7	389	12	3.3 ± 0.2
	413	20		421	14
	419	17		456	16
BGL-1	567	41	9.1 ± 1.8	512	25	4.3 ± 0.5
	418	45		504	20
	421	39		462	19
Naphthylacetic acid (3.0) + zeatin-O-glucoside (1.0)
UT	501	18	4.1+0.5	512	18	3.4+0.4
	402	19		461	17
	479	20		470	14
BGL-1	510	132	27.5 ± 2.2	439	52	11.4 ± 1.0
	408	122		481	59
	412	110		477	49
Zeatin (1.0)
UT	512	35	6.8 ± 0.9	514	23	4.6 ± 0.3
	427	32		525	26
	501	30		426	19
BGL-1	433	55	10.6 ± 0.8	421	28	6.2 ± 0.4
	479	49		513	31
	534	48		401	24
Zeatin-O-glucoside (1.0)
UT	521	0	0	458	0	0
	520	0		421	0
	524	0		521	0
BGL-1	551	36	7.5 ± 1.2	471	18	3.7 ± 0.3
	478	42		440	15
	499	37		502	20

^aNumber of protoplasts dividing per number of total protoplasts in the same visual field of the microscope.

^bNumber of protoplasts dividing and forming cell groups per number of total protoplasts in the same visual field of the microscope.