Figure 1.

Generation of ACO- and ACS/ACO-silenced plants. A, Structure of the OsACS2, OsACO5, and OsACO7 genes and schematic representation of the constructs used to transform rice plants. Boxes below individual cDNAs indicate the regions used for construction. B, Emission of ethylene from leaves after wounding. Leaves were cut into small pieces and incubated in a sealed vial at 25°C for 24 h under continuous light. A transgenic IL7 line transformed with the vector alone was used as a control (Cont.). C, Reverse transcription-PCR analysis of rice ACS and ACO genes in leaves at 24 h after wounding. OsActin3, which is constitutively expressed in leaves, was used as an internal control. D, Quantitative real-time PCR analysis of the OsACO1 gene in leaves at 48 hpi. Values are means ± se (n = 12).