Figure 3.

Breakdown of Pi-i-originated blast resistance in the leaf sheath of ACO/ACS knockdown rice plants. The inner sides of intact leaf sheaths of each transgenic IL7 line were inoculated with a suspension of blast conidia and incubated at 25°C in a humidified transparent box. After 5 d, the inoculated leaf sheaths were observed using a microscope. A, Left, Inoculated leaf sheaths at 5 dpi. Right, Schematic representation of lesions in the left section. Black arrowheads and black arrows indicate DBLs and BLs, respectively. Bar indicates 5 mm. B, Photograph of leaf sheath around DBLs in control and that around BLs in IRACS/ACO13 lines. Left, A close-up view of a lesion highlighted using red lines in the right section. In the control, necrotized mycelia were detected (white arrows). In the IRACS/ACO13 line, transparent mycelia (red arrows) and clumps of the mycelia, which are highlighted using red dashed circles, were observed. Representative images are shown. Ap, Appressorium. Bar indicates 50 μm.