Exogenous ethylene has no effect on induction of blast resistance. A, Timeline of the assay with detached leaves. Detached leaves were treated with water (Control), ethylene, or a 0.5 mm solution containing ethephon, KCN, ACC, MJ, or SA in a sealed tube. B, SLs observed in the leaves at 4 dpi after the treatment of lactophenol-trypan blue. Bar indicates 10 mm. The number (C) per leaf of SLs more than 0.5 mm2 and their mean area (D) were determined. Values for C and D are means ± se (n = 5).