Table II. A typical purification of the 6-SST/6-SFT from P. terminalis rhizomes.
Fraction | Protein | Activity | Recovery | Specific Activity | Purification |
mg | units | % | units (mg protein)−1 | fold | |
Crude extract | 162 | 3.9 | 100 | 0.024 | 1.0 |
(NH4)2SO4 30%–80% | 151 | 3.7 | 95 | 0.025 | 1.0 |
Concavanalin A | 5.3 | 2.5 | 64 | 0.48 | 20 |
Mono S 4.5 | 0.92 | 1.5 | 38 | 1.6 | 67 |
Mono S 4.0 | 0.17 | 0.35 | 8.8 | 2.1 | 88 |