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. 2011 Jan 3;155(1):447–463. doi: 10.1104/pp.110.164848

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© 2011 American Society of Plant Biologists

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Figure 2. — Binding of CaMBL1 to d-Man. A, Schematic representation of CaMBL1 and CaMBL1 deletion constructs. B, Immunoblot analysis of CaMBL1 using the anti-His antibody. His-tagged CaMBL1 and CaMBL1 deletion constructs were expressed in E. coli (left panel) and purified using d-Man-agarose columns. Arrowheads indicate expressed His-tagged recombinant proteins. aa, Amino acids; CaMBL1-1, signal peptide deleted; CaMBL1-2, GNA-related lectin domain only; CaMBL1-3, C-terminal region only; GNA domain, GNA-related lectin domain; His, His tag; SP, signal peptide region; α-His, anti-His antibody; IP, immunoprecipitation; M, molecular mass markers; WB, western blotting. [See online article for color version of this figure.]