Figure 4.

Transient expression of cell death in pepper leaves infiltrated with Agrobacterium GV3101 carrying the 35S:00 (empty vector) or 35S:CaMBL1 construct. A, Visible and UV light-illuminated phenotypes of CaMBL1-transiently expressing leaves 4 d after infiltration with different bacterial concentrations (OD₆₀₀). B, Expression of CaMBL1 protein in empty vector control leaves and 35S:CaMBL1 leaves as detected by immunoblotting. Coomassie Brilliant Blue (CBB) staining is shown for the 60-kD regions of protein extracts. C, Staining with trypan blue of leaf tissues 1 d (right) and 2 d (left) after infiltration. Bars = 0.5 mm. D, Electrolyte leakage assay of CaMBL1-transiently expressing leaves at different times after agroinfiltration. E, Real-time quantitative PCR analysis of CaBPR1 and CaDEF1 in CaMBL1-transiently expressing leaves. Transcript levels were normalized to the expression of pepper 18S ribosomal RNA measured in the same samples. F, Levels of SA in CaMBL1-transiently expressing leaves. All experiments were repeated three times with similar results. Values are presented as means ± sd. Asterisks indicate significant differences between the means, as determined by Student’s t test (P < 0.05). FW, Fresh weight; H, healthy plants. [See online article for color version of this figure.]