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. 2011 Jan 3;155(1):447–463. doi: 10.1104/pp.110.164848

Figure 5.

Figure 5.

Agrobacterium-mediated transient expression of 35S:00 (empty vector), 35S:CaMBL1, 35S:GNA domain, and 35S:CaMBL1c in pepper leaves. A, Visible and UV light-illuminated phenotypes of the 35S:00 (empty vector), 35S:CaMBL1, 35S:GNA domain, and 35S:CaMBL1c-transiently expressing pepper leaves 4 d after agroinfiltration. B, Immunoblot analysis of 35S:8cMyc, 35S:CaMBL1:8cMyc, 35S:GNA domain:8cMyc, and 35S:CaMBL1c:8cMyc protein expression in pepper leaves using an anti-cMyc antibody. Coomassie Brilliant Blue (CBB) staining is shown for the 60-kD regions of protein extracts. C, Electrolyte leakage assay of 35S:00 (empty vector), 35S:CaMBL1, 35S:GNA domain, and 35S:CaMBL1c-transiently expressing pepper leaves at different times after agroinfiltration. All experiments were repeated three times with similar results. Values are presented as means ± sd. Different letters indicate significant differences as determined by Fisher’s lsd test (P < 0.05).